EPMA observation between dentin and resin interfaces. Part 1. Comparison of wet and dry technique after short-term stored in water.

The study was to evaluate the marginal leakage of wet or dry dentin condition after restoration on short-term. In the study we used Photobond adhesive system and Single Bond adhesive system. The specimens were prepared from premolars. A class V cavity was prepared at the CEJ with a high-speed hand piece and #010 round diamond point. The cavity's walls and floors were etched with phosphoric acids of the test materials by wet and dry bonding techniques. The specimens were cross-sectioned longitudinally through the center of the cavities with a low speed diamond micro-cutter and polished with carbide paper (#600-1200) after storage in distilled water for 1 day, and silver nitrate staining. Specimens were viewed with EPMA for elemental distribution of calcium, nitrogen and silver on the resin-dentin interface. The uptake of silver particles was less in samples treated with the wet-bonding technique when compared with dry-bonding technique. This in vitro study showed that bonding technique is important in establishing a seal along the restoration margins to control marginal leakage.